rnascope chromogenic in situ hybridization (cish)  (Advanced Cell Diagnostics Inc)

Journal: eBioMedicine

Article Title: B4GALNT3 regulates glycosylation of sclerostin and bone mass

doi: 10.1016/j.ebiom.2023.104546

Figure Lengend Snippet: B4galnt3 is expressed in osteoblasts and osteocytes but not in osteoclasts. a–l) Representative in situ hybridization images in mice. Transverse sections across lumbar vertebra 5 (a–d) and longitudinal sections of femur or tibia (e–l) in mice demonstrate the mRNA expression of B4galnt3 (blue; a–l) and Sost (red; a, e, i), Dmp1 (red; b, f, j), Runx2 (red; c, g, k) or Ctsk (red; d, h, l). B4galnt3 mRNA could be observed in Sost-, Dmp1-, and Runx2-expressing osteocytes and osteoblast- lineage cells on the bone surface. In contrast, B4galnt3 was not detectable in Ctsk-expressing osteoclasts. Scale bar 50 μm. Images are representative of at least three independent experiments.

Article Snippet: In situ hybridization The chromogenic in situ hybridization (cISH) was performed by using the RNAscope Duplex Detection Kit (322500, Advanced Cell Diagnostics (ACD), Bio-Techne Ltd., Abingdon, UK).

Techniques: In Situ Hybridization, Expressing

Journal: Cells

Article Title: Molecular and Kinetic Analyses of Circulating Tumor Cells as Predictive Markers of Treatment Response in Locally Advanced Rectal Cancer Patients

doi: 10.3390/cells8070641

Figure Lengend Snippet: Immunostaining ( A–C ) and chromogenic in situ hybridization (CISH) ( D–F ) of CTCs from locally advanced rectal cancer (LARC) patients. ( A ) CTCs and leukocytes visualized with haematoxylin-eosin staining (×40 magnification). ( B ) CTCs stained with an anti-thymidylate synthase (TYMS) antibody, visualized with Permanent Red, and counterstained with haematoxylin (×40 magnification). ( C ) CTCs stained with an anti-RAD23B antibody, visualized with 3,3-diaminobenzidine (DAB), and counterstained with haematoxylin (×60 magnification). ( D ) CTCs negative for TYMS mRNA and counterstained with haematoxylin (×40 magnification). ( E ) CTCs with a low TYMS mRNA signal (normal expression) counterstained with haematoxylin (×40 magnification). ( F ) CTCs with a high TYMS mRNA signal (overexpression) counterstained with haematoxylin (×40 magnification). All images were analyzed on a Research System Microscope BX61 (Olympus, Tokyo, Japan) coupled to a digital camera (SC100–Olympus). Thick arrows indicate CTCs, thin arrows indicate leukocytes, and asterisks indicate 8 μm pores of the ISET ® membranes.

Article Snippet: TYMS mRNA was detected in intact cells by using a chromogenic in situ hybridization (CISH) assay employing RNAscope Technology (ACDbio, Newark, CA, USA), according to the manufacturer’s protocol.

Techniques: Immunostaining, Chromogenic In Situ Hybridization, Staining, Expressing, Over Expression, Microscopy

Journal: Cells

Article Title: Molecular and Kinetic Analyses of Circulating Tumor Cells as Predictive Markers of Treatment Response in Locally Advanced Rectal Cancer Patients

doi: 10.3390/cells8070641

Figure Lengend Snippet: Expression profiles of RAD23B and TYMS proteins and TYMS mRNA.

Article Snippet: TYMS mRNA was detected in intact cells by using a chromogenic in situ hybridization (CISH) assay employing RNAscope Technology (ACDbio, Newark, CA, USA), according to the manufacturer’s protocol.

Techniques: Expressing

Journal: Cells

Article Title: Molecular and Kinetic Analyses of Circulating Tumor Cells as Predictive Markers of Treatment Response in Locally Advanced Rectal Cancer Patients

doi: 10.3390/cells8070641

Figure Lengend Snippet: Scheme demonstrating both methodologies used to select responders and non-responders to neoadjuvant chemoradiotherapy: mRNA detected by CISH and protein expression detected by immunocytochemistry (ICC).

Article Snippet: TYMS mRNA was detected in intact cells by using a chromogenic in situ hybridization (CISH) assay employing RNAscope Technology (ACDbio, Newark, CA, USA), according to the manufacturer’s protocol.

Techniques: Expressing, Immunocytochemistry